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Molecular Manipulation & Analysis Lasers
What are Molecular Manipulation & Analysis Lasers?
Molecular manipulation & analysis lasers in the field of biophotonics are specialized lasers (typically CW) used for studying and manipulating individual cells in biological systems. These lasers provide precise control and targeted energy delivery to enable various cellular analysis and manipulation techniques, providing researchers with powerful tools to explore cellular processes, understand cellular functions, and investigate disease mechanisms. These lasers offer precise control, high resolution, and versatility, enabling advancements in areas such as cell biology, neuroscience, regenerative medicine, and drug discovery. Common applications within this group include confocal fluorescence microscopy, DNA sequencing, flow cytometry, optogenetics, and more.
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Molecular Manipulation & Analysis Applications
Confocal Fluorescence Microscopy Lasers: Confocal microscopy is an optical technique that allows for extremely high spatial resolution imaging by placing two conjunct pinholes in the optical path. Most of the visible wavelengths can be used in confocal fluorescence microscopy, but it’s essential that all confocal fluorescence microscopy lasers (typically CW, low noise, single-mode, visible) have a high quality TEM00 beam profile.
DNA Sequencing Lasers: DNA sequencing or genome sequencing is an extremely powerful tool that allows the identification of the four basic DNA molecules adenine (A), cytosine (C), guanine (G), and thymine (T) by tagging them with fluorescence reporter molecules. Like all fluorescence applications, most of the visible wavelengths can be used in DNA sequencing, but it’s essential that all DNA sequencing lasers (typically CW, low noise, single-mode, visible) have a high quality TEM00 beam profile.
Flow Cytometry Lasers: Flow cytometry is a laser-based technology employed in cell counting, cell sorting, biomarker detection, and protein engineering. This technology is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Flow cytometry lasers (typically CW, low noise, single-mode, visible or IR) provide a high-quality TEM00 beam profile, low noise, and high pointing stability and power stability.
Optogenetics Lasers: Optogenetics is a biological technique that uses light to control cells in living tissue, typically neurons. While very little optical power is needed, optogenetics lasers (typically ns ps or fs pulsed, visible) are still the preferred delivery method because of their ability to be focused and targeted to specific areas of the brain. Additionally, several wavelengths in the near-infrared region allow optogenetics lasers to be transmitted through bone and tissue eliminating the need for opening the skull.
Two-Photon Excitation Microscopy: Often called two-photon microscopy (2-photon, multi-photon, TPE microscopy), this process creates fluorescence images of live cells utilizing ultrashort pulsed lasers (typically mode-locked, fs pulsed lasers with NIR wavelengths). Compared to the usual one-photon microscopy, this method offers many benefits, such as better signal-to-noise ratio for clearer images, reduced photobleaching and phototoxicity, and deeper penetration in dense tissues.
Recommended Laser Series
We recommend the following laser series options for Molecular Manipulation & Analysis applications. There can often be a ton of options and many variables. Contact us today for help finding the perfect laser for your specific application!
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The Halite 2 series is a compact, single-box, all-fiber femtosecond laser designed to meet the most demanding applications in neuroscience, biophotonics, microscopy, and engineering. With pulses as short as <250 fs (< 100 fs op.), average power over 2 W at 1030 nm, and the option of second harmonic at 515 nm, it is an irreplaceable tool in every lab that needs a reliable, turn-key, ultrafast light source. Thanks to its unique construction and SESAM-free technology, it is a cost-effective solution that provides high pulse energy (over 100 nJ), excellent beam quality, and easy integration.
The Jasper series is a diverse set of high-performance femtosecond fiber lasers providing different solutions based on a standard technology. The series includes the high-power Jasper X0 (200 µJ pulse energy / 60 W average power), the compact Jasper Micro (5 µJ pulse energy / 7 W average power) in a space-saving design, and the Jasper Flex for microprocessing (30 µJ pulse energy). Each model provides a fast warm-up time, long-term stability, and hands-free operation. Whether you need high power, compactness, or specific processing capabilities, the Jasper series has a solution.
The Q-TUNE-HR series is a high repetition rate OPO producing a tunable wavelength range from ≈ 750 – 1800 nm and up to 100 kHz pulse repetition rate, the perfect coherent light source for micromachining, Raman spectroscopy, microscopy, and remote sensing applications. Q-TUNE-HR requires an external pump source with > 350 μJ pulse energy and 5 – 7 ns FWHM pulse duration @ 532 nm for operation: pulse repetition rate can be in 10 – 100 kHz range and acceptable pump power in the 3.5 – 30 W range. By default OPO is optimized to produce maximum output in 1200 – 1800 nm range (optional: 750 – 950 nm). In addition to the tunable range, the pump laser wavelength is accessible through a convenient bypass port.
The Q-TUNE-IR series is the perfect high peak power, coherent DPSS light source for researchers with infrared spectroscopy applications. This series uses an Optical Parametric Oscillator (OPO) to produce a tunable wavelength of 1380–4500nm (<10 cm-1 linewidth), achieving > 15mJ of pulse energy at the peak of the tuning curve. The Q-TUNE-IR requires little maintenance, with all laser electronics integrated into the housing, including an air-cooling system, eliminating the need for chillers or large power supplies while providing a guaranteed > 2 G shot pump diode lifetime.
The SL-Pico series of picosecond supercontinuum lasers is designed to meet the diverse and dynamic needs of cutting-edge research and industrial applications. These supercontinuum white lasers are highly regarded for their wide wavelength range and cost-effectiveness. The SL-Pico offers a spectral range from 410 to 2400 nm, has high power, is very stable, and is capable of delivering power up to 8 W. The SLM versions are mode-locked fiber lasers with a fixed rep. rate, and the SLMV versions have a tunable repetition rate (up to 40 or 200 MHz), ensuring compatibility with a wide range of devices and various applications like fluorescence microscopy, TCSP, hyperspectral imaging, semiconductor inspection, and much more!
The TLS series is the broadest continuously optically tunable broadband picosecond laser combining a supercontinuum laser & tunable bandpass filter. Users can tune output power, wavelengths from 410-1700nm by choosing the VIS, IR, SWIR, or a custom configuration, and real-time bandwidth control for TLS-Red (10 or 20nm fixed for TLS-Blue). These picosecond tunable lasers are suitable for various fields that require precision scanning and high output from fluorescence microscopy to time-resolved spectroscopy, such as TCSPC, Hyperspectral imaging, Machine vision, Semiconductors, Sensors, and other applications.