What type of laser do I need for confocal microscopy?

The short answer is: You have some flexibility, but the laser source should be PM fiber-coupled and have a low noise, TEM₀₀ beam mode. The excitation bandwidth of the fluorophores used must overlap with the laser wavelength, as various fluorophores need different wavelengths. So, you may require multiple lasers, which means you’ve got a beam combining alignment challenge to tackle. One way to avoid this is through the convenience of Multi-Wavelength Beam Combiners.

If you want to learn more on the subject of confocal fluorescence microscopy, ideal laser sources, and the benefits of beam combiners, check out this white paper: “Multi-Wavelength Laser Sources for Multi-Color Fluorescence Microscopy.” Get more information from our Lasers 101, Blogs, Whitepapers, FAQs, and Press Release pages in our Knowledge Center!