Multi-Wavelength Combiners or laser beam combiners allow you easily and efficiently combine multiple wavelength laser beams into one (or more) convenient outputs. The technique, called spectral beam combining (wavelength beam combining, incoherent beam combining), takes multiple different wavelength laser sources and utilizes a wavelength-sensitive beam combining optic, like a prism, diffraction grating, or dichroic mirror, to combine the beam paths and direct them to a common output.
CW multi-wavelength combiners have proven to be a critical component in Life Science applications, reducing time, cost, and complexity, and fundamentally changing these applications, allowing for increased efficiency and ease of use. Given the immense degree of complexity and time involved with combining multiple laser beams into a confocal microscope, multi-wavelength combiner modules have flooded into the market in recent years.
These plug-n-play style modules allow for swappable laser modules and quick and easy coupling with a confocal microscope, saving hours in precision alignment efforts. Laser combiner modules also help to avoid further compounding the complexity of the process, resulting from trying to select multiple different wavelength sources from various laser models and even from various manufacturers, likely with differing specifications. With a huge selection of wavelength combiners on the market, picking the right solution for your needs can be a difficult task.
Our Multi-Wavelength Combiner Products
L6Cc & L4Cc
The L6Cc and L4Cc laser beam combiners are the most compact and flexible all-in-one multi-color laser sources. Available with up to 7 laser lines input and delivering up to 4 optical fiber outputs. The modular design allows for a large choice of lasers from 375 nm up to 1064 nm and with output power up to 500 mW. The laser sources are Oxxius LaserBoxx platform. The extension modules provide the ultimate level of flexibility by integrating fast switching output ports for FRAP, adjustable split power for light sheet microscopy among other advanced functionalities.
The L6Cc and L4Cc are field upgradeable. Therefore, you can easily evolve as your needs change to preserve your investment. Furthermore, they are microprocessor controlled to provide unique features for demanding applications. The L6Cc and L4Cc are available in turnkey or OEM versions. The L6Cc is also available in high power version, integrating LBX-HPE sources with up to 1.2 W output power per line. Finally, it is coupled into a speckle-free multimode fiber.
Our MatchBox series boasts the World’s Smallest footprint for multi-wavelength combiners. Ideal for portable, handheld applications, these flexible, turn-key combiners, powered by USB, are perfect for integration into battery operated devices. With many laser diode wavelength options from Violet (405nm) to short-wave infrared (SWIR – 1550nm), including RGB options, you choose the precise combination for your application. For example, these RGB laser combiners pair perfectly with the MatchBox series of laser modules: Red wavelengths @ 633nm, 638nm, and 660nm, Green wavelengths @ 505nm and 520nm, and Blue wavelengths @ 450nm, and 488nm.
3 Important Things When Choosing a Laser Combiner for Flow Cytometry
1. Wavelength Availability: As we explained above the most critical consideration when choosing a laser combiner for flow cytometry is to ensure that the laser wavelengths line up with the excitation spectra of your anticipated fluorophores. This is why the LaserBoxx can be configured with any combination of the 22 different wavelength laser options that Oxxius offers between 375 nm and 1064nm. It is important to note that many conventional fluorophores have excitation bands which overlap each other, so you must try and pick each wavelength to ensure that it can be used to excite the maximum number for fluorophores. Also, it is essential to keep in mind that for the dichroic mirrors used inside of a laser combiner each wavelength should be at least 10-nm apart. Since most fluorophores have excitation bands larger than 10-nm this is typically not an issue, but if you are working with narrower than usual absorption bands, the minimum separation must be taken into consideration.
2. Beam Quality: Flow cytometry measures the fluorescence emission of individual cells moving through a jet or microfluid cell, which are typically between 1-µm and 15-µm in diameter….
With over 25 years experience providing multi-wavelength combiners to researchers and OEM integrators working in the life sciences and various markets and applications, and 1000s of units fielded, we have the experience to ensure you get the right product for the application. Working with RPMC ensures you are getting trusted advice from our knowledgeable and technical staff on a wide range of laser products. RPMC and our manufacturers are willing and able to provide custom solutions for your unique application.
If you have any questions, or if you would like some assistance please Contact Us here. Furthermore, you can email us at info@rpmclasers.comto talk to a knowledgeable Product Manager.
Alternatively, use the filters on this page to assist in narrowing down the selection of multi-wavelength combiners for sale. Finally, head to our Knowledge Center with our Lasers 101 page and Blogs, Whitepapers, and FAQ pages for further, in-depth reading.
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